How many operating tips do you know about ELISA kits?

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17 Essential Operation Tips for ELISA Kits – How Much Do You Know?

1.

There are various types of ELISA kits available in China, such as ELISA test kits, ELISA kits, enzyme-linked immunosorbent kits, enzyme-linked immunosorbent assay kits, and even enzyme-free kits. The naming can vary, but they all refer to the same kind of technology used for detecting specific antigens or antibodies.

2.

The ELISA kit is designed for the qualitative detection of anti-human hepatitis E (HEV) virus IgM antibodies in human serum or plasma. It's a widely used tool in diagnostic and research settings.

3.

Since its development in the 1960s and 1970s, ELISA has gained global recognition and is now extensively used in laboratories across Europe, America, and Asia. Its reliability and ease of use have made it a staple in biochemistry and immunology.

4.

ELISA is a highly sensitive, specific, and reproducible method for biological testing. With stable reagents, easy storage, and straightforward operation, it’s a preferred choice in many immunological applications.

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So what are the key operational skills you need to master when using an ELISA kit?

Today, we’ll walk you through 17 essential tips that will help you perform ELISA experiments more efficiently and accurately. Whether you're a beginner or an experienced researcher, these guidelines will improve your workflow and reduce experimental errors.

Here are the 17 operation skills summarized by Shanghai Jiwei Biotechnology Co., Ltd.:

1. After adding the enzyme reagent, gently blot the plate surface with a paper towel to dry it properly.

2. Plan the number of samples to be tested in advance to avoid long waiting times during washing steps.

3. When aspirating liquid, use a pipette that closely matches the required volume to minimize error.

4. Always perform duplicate tests to ensure data accuracy and reflect the kit's precision.

5. Use a dosing device to fill the sample diluent and regularly calibrate it for accuracy.

6. To prevent evaporation, place the reaction plate in a closed container covered with a damp cloth, and cover the plate with a lid or film.

7. Store unused plates and reagents at 2–8°C. The horseradish peroxidase-labeled anti-human IgG working solution should be used immediately after preparation and not reused.

8. After adding the sample, place the incubator promptly. If there are many samples, process them in batches and strictly follow the instructions to avoid over-incubation, which may cause non-specific binding.

9. Sterilize any remaining samples and waste by autoclaving at 121°C for 30 minutes or treating with 5.0 g/L sodium hypochlorite for 30 minutes.

10. During washing, ensure each well is fully filled with wash buffer to avoid residual enzymes from being washed away.

11. Leave one well as a blank control for zero adjustment. No additional reagents are added except for the substrate and 2N H2SO4.

12. After manual washing, let the wash buffer sit for 15–30 seconds before removing it. Avoid splashing between wells to prevent cross-contamination. Dry the plate on absorbent paper after washing.

13. If results are uncertain, consider verifying them with alternative methods.

14. If deionized or distilled water is unavailable, use Wahaha purified water. Never use tap water for solutions.

15. Replace pipette tips when transferring liquids between different bottles, even when handling standard solutions.

16. Avoid rapid aspiration to prevent air bubbles and inaccurate measurements.

17. Use a pipette that matches the required volume to reduce measurement errors.

Shanghai Jiwei Biotechnology Co., Ltd. is a leading manufacturer of high-quality ELISA kits. Our range covers multiple species including human, mouse, rat, hamster, guinea pig, pig, bovine, sheep, canine, and plant. We are committed to providing reliable and accurate products for researchers worldwide.