Elisa kit technology to explore the competition law steps - Database & Sql Blog Articles

Test probe P100-M3

EL-C1600N100013-B

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Kaixin micro test

Our ELISA kits are built on solid scientific principles. The core of immunoassay technology lies in the specific interaction between antigens and antibodies. Therefore, the quality of any diagnostic reagent heavily depends on the quality of its raw materials—such as antigens, antibodies, and enzymes. When a small molecule antigen or a partial antibody lacks two or more binding sites required for a sandwich assay, it cannot be measured using the standard double-antibody sandwich method. In such cases, a competitive assay is typically used.

The basic principle of the competitive method involves a competition between the target antigen in the sample and an enzyme-labeled antigen for binding to a limited number of antibody sites. After washing, the samples are divided into two groups: one group contains both the tested antigen and the enzyme-labeled antigen, while the other group only contains the enzyme-labeled antigen. Following incubation and substrate addition, a color reaction occurs. By comparing the intensity of the color between the experimental and control groups, we can determine the concentration of the unknown antigen in the sample.

This technique is widely used in ELISA assays for small molecules like hormones and drugs, where traditional sandwich methods may not be applicable.

Brief Steps of the Competitive Method:

• 1) First, an antibody is immobilized onto the surface of the microplate.
• 2) A mixture of the sample and enzyme-labeled antigen is added, along with a control group containing only the enzyme-labeled antigen.
• 3) After incubation, the substrate is added, triggering a color reaction. The difference in color intensity between the experimental and control groups is used to calculate the amount of the unknown antigen.

We take great care in selecting high-quality imported antibodies, standards, chromogenic reagents, and ELISA plates to ensure optimal performance. Our products are developed using advanced patented technologies and are manufactured strictly according to standardized production procedures. We import key raw materials in batches and use our own special formulation diluents to maintain consistent quality while keeping costs under control. If you're interested in learning more or placing an order, feel free to contact us today.